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Specific to the portion of Human Histone H2AX phosphorylated at Ser139. Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation. Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci. A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry.

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Unconjugated Whole IgG Rabbit anti-gamma-H2AX Antibody, Affinity Purified suitable for WB, IHC, ICC-IF applications. Visit Bethyl.com for all your antibody needs. Bethyl is now a part of Fortis Life Sciences. induce its function.

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Clonality. Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples.

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Host Rabbit. Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal (H2AX Ser139 p ); HEK 293 cells, monoclonal RM224, purified immunoglobulin; Suitable for immunocytochemistry; Histone H2AX (H2A histone family member X), a histone variant present in almost all eukaryotes, is coded by H2AFX gene; Evaluate Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci. PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. By tagging gamma-H2AX histones with a fluorescent antibody, Dr. Bonner showed that exposing cells to ionizing radiation caused hundreds of H2AX histones near the site of a double-strand break to become phosphorylated within minutes, creating a rapid and highly amplified response akin to a cellular fire alarm.

Gene Synonyms. H2AX, H2a.x, H2afx, H2A/X. 2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin I have tried 53bp1 (MAB 3802) from millipore and gamma h2ax (ab11174) from Abcam with 3.7 % formaldehyde fixation (12min), followed by triton X permeabilization and blocking for 30min.
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A300-081A 0.1 ml. 4 Nov 2016 X, Gamma-H2AX; Isotype: Mouse IgG1, κ; Ave. Rating The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and  9 Jun 2020 antibodies specific for phosphorylated H2AX6.

Applications WB, IHC, ICC-IF. Host Rabbit. Histone H2A.X Antibodies Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX.
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Measuring DNA Damage and Repair in Mouse Splenocytes

Exp Cell Res 300, 320–334 (2004). CAS Article 2017-02-03 · The combined γ-H2AX/H2AX ELISA is performed by coating different wells of the same plate (or two different plates) with 2 different capture antibodies, each specific for a different part of H2AX: an anti-H2A antibody able to bind all histone H2A types, including H2AX, and γ-H2AX antibody able to bind only the phosphorylated form of H2AX (serine 139). Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several (NB100-79967) gamma H2AX [p Ser139] Antibody (EP854(2)Y) This product has been discontinued by Novus Biologicals, but remains here on CiteAb for record purposes.